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KMID : 1036320200420010023
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Maxillofacial Plastic and Reconstructive Surgery
2020 Volume.42 No. 1 p.23 ~ p.23
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4-Hexylresorcinol induced angiogenesis potential in human endothelial cells
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Kim Min-Keun
Kim Seong-Gon
Lee Suk-Keun
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Abstract
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Background: 4-Hexylresorcinol (4HR) is able to increase angiogenesis. However, its molecular mechanism in the human endothelial cells has not been clarified.
Methods: As endothelial cells are important in angiogenesis, we treated the human umbilical vein endothelial cells (HUVECs) with 4HR and investigated protein expressional changes by immunoprecipitation high-performance liquid chromatography (IP-HPLC) using 96 antisera.
Results: Here, we found that 4HR upregulated transforming growth factor-β (TGF-β)/SMAD/vascular endothelial growth factor (VEGF) signaling, RAF-B/ERK and p38 signaling, and M2 macrophage polarization pathways. 4HR also increased expression of caspases and subsequent cellular apoptosis. Mechanistically, 4HR increased TGF-β1 production and subsequent activation of SMADs/VEGFs, RAF-B/ERK and p38 signaling, and M2 macrophage polarization.
Conclusion: Collectively, 4HR activates TGF-β/SMAD/VEGF signaling in endothelial cells and induced vascular regeneration and remodeling for wound healing.
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KEYWORD
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4HR, HUVEC, IP-HPLC, TGF-β1, Angiogenesis
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